2016 Fiscal Year Final Research Report
Direct visualization of endogenous gene expression through targeted genetic labeling
| Project/Area Number |
26250001
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
近藤 周 国立遺伝学研究所, 系統生物研究センター, 助教 (90408401)
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| Research Collaborator |
YAMAGATA Nobuhiro 東北大学, 大学院生命科学研究科, 助教 (50716248)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 脳機能モデル動物 / 遺伝学 |
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| Outline of Final Research Achievements |
Comprehensive connectivity maps of nervous systems and connectomics are providing detailed designs of neuronal circuits. Although these connectivity maps are powerful in predicting hypothetical circuit functions and guiding new experiments thereon, the information of neurotransmitters and receptors is critical in interpreting how a given circuit functions. This project research aims at comprehensive mapping of neurotransmitters in the Drosophila brain. Using the CRISPR/Cas9 system, we generated a transgenic library of GAL4 knock-in that recapitulate the expression of genes for neurotransmitter biosynthesis and their receptors. By visualizing their expression patterns in the brain, we quantitatively compare the distribution of neurotransmitter-releasing and receiving cells. This study provided the basis of neurotransmitter map in the fly brain.
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| Free Research Field |
神経行動学
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