Molecular mechanism of H3K9me-mediated transcriptional silencing

2017 Fiscal Year Final Research Report

• Project/Area Number: 26250039
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genome biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Shinkai Yoichi 国立研究開発法人理化学研究所, 眞貝細胞記憶研究室, 主任研究員 (20211972)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: CRISPR/Cas9 / レトロエレメント / 転写抑制 / SETDB1 / 網羅的KOスクリーニング / H3K9メチル化 / ES細胞

Outline of Final Research Achievements

The Moloney murine leukemia virus (MLV)-based retroviral vector MSCV-GFP, which is repressed by the histone H3 lysine 9 methyltranferase SETDB1 pathway in mESCs was used as a reporter provirus, we performed a genome-wide CRISPR screen to advance our understanding of retroelement silencing in mESCs. We identified more than 80 genes involved in this process including not only already known retroelement silencing genes but also multiple novel ones. Among the characterized novel factors, we find that they seem to work for silencing in different layers, thus upstream and parallel or downstream of SETDB1.

Free Research Field

分子生物学