Molecular mechanism of chromatin remodeling coupled with DNA transaction

2016 Fiscal Year Final Research Report

• Project/Area Number: 26251008
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto University (2016); International Institute for Advanced Studies (2014-2015)
• Principal Investigator: Morikawa Kosuke 京都大学, 生命科学研究科, 研究員 (80012665)
• Co-Investigator(Kenkyū-buntansha): 津中 康央 京都大学, 物質-細胞統合システム拠点, 特任講師 (40551552)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: DNAトランスアクション / クロマチンリモデリング / 構造生物学

Outline of Final Research Achievements

We assumed that the interaction of chromatin remodeling factors with nucleosomes would involve local detachments of the nucleosomal DNA from histones during DNA transaction. Therefore, we studied the interaction of chromatin remodeling factors with nicked nucleosomes by biochemical and structural analyses. As a result, the introduction of a double-strand break (DSB) into the nucleosomal DNA allows a histone chaperone FACT to efficiently form a complex with nucleosomes. It is thus indicated that DNA cleavages on nucleosomes would cause local unwrapping of the nucleosomal DNA in the vicinity of the cleavage sites upon contacting FACT, leading to subsequent reactions for chromatin remodeling.

Free Research Field

構造生物学