2016 Fiscal Year Final Research Report
Mechanism and Evolution of RNA polymerases
| Project/Area Number |
26251009
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | The University of Tokyo (2016)
National Institute of Advanced Industrial Science and Technology (2014-2015) |
|---|
Principal Investigator |
Tomita Kozo 東京大学, 大学院新領域創成科学研究科, 教授 (00345274)
|
|---|
| Project Period (FY) |
2014-06-27 – 2017-03-31
|
| Keywords | RNA / 構造 / 反応機構 |
|---|
| Outline of Final Research Achievements |
In this project, we focus on the molecular mechanism of RNA polymerizations by CC-, A- CCA-adding enzymes and by RNA polymerase complexed with S1 protein. We clarified the mechanisms of CC-addition and A-addition by CC-adding and A-adding enzymes, respectively. We also clarify the mechanism by which CCA-adding enzyme discriminates mature tRNA from immature tRNA. We also clarified the molecular basis of replication initiation by using S1 protein in RNA polymerase complex.
|
| Free Research Field |
分子生物学、構造生物学
|
