2017 Fiscal Year Final Research Report
Studies on blue light signaling in stomatal guard cells
| Project/Area Number |
26251032
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 孔辺細胞 / 気孔 / H+-ATPase / 二酸化炭素 / 陰イオンチャネル |
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| Outline of Final Research Achievements |
Using thermography of leaf, we isolated the mutants that exhibited the reduction of stomatal opening in response to blue light. We identified the responsible gene as AHA1, an isoform of the H+-ATPases in guard cells. We also determined the phosphorylated proteins in guard cells in response to blue light utilizing the phosphoproteome analyses. We selected a rapidly phosphorylated protein in phototropin-dependent manners and identified the protein as MAPKKK, and named it as CBC1. We found that CBC1 and its homolog CBC2 redundantly function to stimulate stomatal opening in response to blue light. We clarified that CBCs mediated the inhibition of S-type anion channels in response to blue light. Such inhibition was required for the full-opening of stomata. We also found that CBCs mediated stomatal opening in response to low concentration of CO2. CBCs receive signals from both blue light and CO2, and stimulate photosynthetic CO2 fixation in plant.
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| Free Research Field |
植物生理学
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