2016 Fiscal Year Final Research Report
Autonomous regulatory mechanism of metal ion levels by membrane transport system and metal-binding protein
| Project/Area Number |
26252011
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAWACHI Miki 名古屋大学, 大学院生命農学研究科, 准教授 (40625125)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Membrane / Metals / Membrane transpoter / Ion concentration |
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| Outline of Final Research Achievements |
Plants regulate concentrations of metal ions in order to maintain their activities. We focused on the ion transport systems and ion-sensing machineries and revealed the followings. 1) The cytosolic histidine-rich loop changes its configuration and functions acts as a sensor of cytosolic zinc to maintain an essential level in the cytosol. This was evidence by in vivo examination using loss-of-function mutant plants. 2) New-type zinc transporter MTP12 forms a hetero-oligomer with MTP5 and functions as zinc transporter in the membrane of Golgi apparatus. 3) A newly identified calcium-binding protein PCaP1, which is stably associated with the plasma membrane, changes its conformation by binding with metal ions. The expression of PCaP1 gene is enhanced by increased copper levels and its loss-of-function mutant plants become sensitive to high copper levels. Also, PCaP1 is involved in the closing mechanism of stomata in leaves.
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| Free Research Field |
Biochemistry, Plant Molecular Biology
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