Lignification in polysaccharide matrix mimicking wood cell wall and clarification of lignin structure -control factor

2017 Fiscal Year Final Research Report

• Project/Area Number: 26252022
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Wood science
• Research Institution: Hokkaido University
• Principal Investigator: Uraki Yasumitsu 北海道大学, 農学研究院, 教授 (90193961)
• Co-Investigator(Kenkyū-buntansha): 重冨 顕吾 北海道大学, 農学研究院, 講師 (20547202) ; 玉井 裕 北海道大学, 農学研究院, 准教授 (50281796) ; 吉永 新 京都大学, 農学研究科, 准教授 (60273489) ; 岸本 崇生 富山県立大学, 工学部, 准教授 (60312394) ; 高部 圭司 京都大学, 農学研究科, 教授 (70183449) ; 幸田 圭一 北海道大学, 農学研究院, 講師 (80322840)
• Project Period (FY): 2014-06-27 – 2018-03-31
• Keywords: リグニン / ヘミセルロース / 多糖類マトリックス / 脱水素重合 / 分枝構造

Outline of Final Research Achievements

An aim of this study is to elucidate lignin formation mechanism (lignification), especially influence of hemicelloses, with polysaccharide matrix mimicking wood cell wall structure. Polysaccharide matrix was successfully prepared by the deposition of hemicelluloses, xylan and glucomannan, onto bacterial cellulose. Dehydrogenative polymerization of coniferyl alcohol, which was one of monolignols, was attempted in the presence of such polysaccharide matrix. As a result, the polymerization was accelerated by xylan deposition, but was inhibited by glucomannan.
In addition, the structure of the resultant polymerized product (Dehydronation Polymer: DHP) was investigated on a size-exclusion chromatography equipped with a laser light-scattering detector by the comparison with solution structure of a non-branched lignin model compound that is comprised of only 8-O-4’ linkage. This investigation revealed that DHP had a significant frequency of branched structure.

Free Research Field

木質バイオマス構成成分の生合成過程の解明とその利用