Molecular mechanism mediating estrogen feedback to regulate follicular development and ovulation in domestic animals

2017 Fiscal Year Final Research Report

• Project/Area Number: 26252046
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal production science
• Research Institution: Nagoya University
• Principal Investigator: Tsukamura Hiroko 名古屋大学, 生命農学研究科, 教授 (00212051)
• Co-Investigator(Kenkyū-buntansha): 大蔵 聡 名古屋大学, 生命農学研究科, 教授 (20263163) ; 平林 真澄 生理学研究所, 行動・代謝分子解析センター, 准教授 (20353435) ; 前多 敬一郎 東京大学, 大学院農学生命科学研究科(農学部), 教授 (30181580)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: キスペプチン / GPR54 / GnRH / メタスチン / エストロゲン

Outline of Final Research Achievements

The present study aimed to investigate the mechanism mediating estrogen negative and positive feedback regulating follicular development and ovulation, respectively, in mammals. The study focused on the effects of estrogen on Kiss1 (kisspeptin gene) expression in kisspeptin neurons, and demonstrates that the arcuate (ARC) and anteroventral paraventricular nucleus (AVPV)/ preoptic kisspeptin neurons are targets of estrogen to exert the estrogen negative and positive feedback effects, respectively, in rats, mouse, monkeys, and goats. The study also revealed that specific transcriptional factors are expressed in the both kisspeptin neuronal populations. Further, the present study tried to generate immortalized rat AVPV and ARC kisspeptin neuronal cell lines to investigate molecular mechanism mediating the estrogen effects. As a result, we obtained some kisspeptin neuronal cell line candidates from the AVPV and ARC.

Free Research Field

動物