2017 Fiscal Year Final Research Report
Molecular basis of formation and regulation of insect holocentric chromosome
| Project/Area Number |
26252057
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 昌直 北海道大学, 農学研究院, 助教 (20517693)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 分散型動原体 / 染色体 / カイコ / 遺伝子ネットワーク / クロマチン構造 |
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| Outline of Final Research Achievements |
Lepidopteran insects are known to have specific chromosomes called heliocentric chromosome having multiple centromeres along the entire length. In this study, we clarified the molecular basis of kinetochore formation of the heliocentric chromosome and newly found six centromere-related genes specific to silkworm using the gene network analysis. Based on these results and analysis on replication related genes, an artificial chromosome functioning by tethering DNA replication factor and kinetochore protein was synthesized.
On the other hand, as for the genome-wide gene expression regulation and molecular basis of chromatin structure, we focused on histone acetylation and deacetylation enzymes, and performed gene knockdown on these genes. As a result, we found that HDAC 8 plays an important role in the silkworm.
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| Free Research Field |
昆虫ゲノム科学
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