Improved detection of atmospheric bioaerosols by integrating online fluorescence method with offline measurements based on DNA staining

2016 Fiscal Year Final Research Report

• Project/Area Number: 26281018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental dynamic analysis
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: KANAYA Yugo 国立研究開発法人海洋研究開発機構, 地球表層物質循環研究分野, 分野長代理 (60344305)
• Co-Investigator(Kenkyū-buntansha): 宮川 拓真 国立研究開発法人海洋研究開発機構, 地球表層物質循環研究分野, 研究員 (30707568) ; 竹谷 文一 国立研究開発法人海洋研究開発機構, 地球表層物質循環研究分野, 主任研究員 (50377785)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 大気現象 / 生物起源有機エアロゾル / PM2.5 / 蛍光法 / DNA

Outline of Final Research Achievements

Organic particles consist 20-50% of PM2.5 aerosol mass concentrations in the Earth's atmosphere. However, their origins are mostly not well identified. In this study, criteria to identify bioaerosols, a possibly important class of unidentified organics, were established for an online sensor detecting autofluorescence (WIBS4), by comparison to a more reliable, offline measurement with DNA staining. Specifically, comparison measurements at Fukue Island, western Japan, and those during three research cruises on R/V Mirai were conducted and the results were comprehensively analyzed. Optimization of the criteria was made separately for terrestrial and marine origin air masses. We also found that bioaerosols from Siberian forest fires even reached open ocean on the Bering Sea, and also that marine atmospheric bioaerosol concentrations could depend on wind speed and SST, in addition to their corresponding concentrations in the surface seawater.

Free Research Field

大気化学