Creation of Innovative Methodology for Analyzing in vivo Ambiguous Bioactivity of Anti-oxidative Substance to Support the Development of Health and Longevity-promoting Foods

2016 Fiscal Year Final Research Report

• Project/Area Number: 26282025
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Kyushu University
• Principal Investigator: Fujimura Yoshinori 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (20390304)
• Co-Investigator(Kenkyū-buntansha): 兵藤 文紀 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (10380693) ; 早川 英介 九州大学, 先端融合医療レドックスナビ研究拠点, 特任助教 (20739809) ; 瀬戸山 大樹 九州大学, 大学病院, 助教 (30550850) ; 三浦 大典 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: レドックス / 抗酸化活性 / 質量分析イメージング / 磁気共鳴画像診断 / 代謝 / 酸化ストレス / 緑茶カテキン

Outline of Final Research Achievements

Herein, we could non-invasively visualizing the redox status of the tissue region targeted by anti-oxidative food factors in redox-related disease mice by using redox molecular imaging (ReMI) technique. In addition, mass spectrometry imaging (MSI) technique allowed us to visualize the tissue distribution of the representative redox-sensitive probe as a redox contrast agent. Combination of the two techniques succeeded in an integrative analysis of more detailed redox and metabolic statuses on the basis of simultaneous evaluation of such imaging data and both imaging data from endogenous metabolome and redox-related biological indices. Furthermore, a food factor, with higher anti-oxidative activity in vitro, did not significantly affect in vivo redox status. These findings provide the useful and essential information to accurately understand in vivo mechanism of food factors.

Free Research Field

食品機能学、メタボロミクス