Development of a reagent-less urine components monitor based on near-infrared spectroscopy.

2018 Fiscal Year Final Research Report

• Project/Area Number: 26282119
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kanazawa University
• Principal Investigator: Tanaka Shinou 金沢大学, フロンティア工学系, 教授 (40242218)
• Co-Investigator(Kenkyū-buntansha): 小川 充洋 帝京大学, 理工学部, 准教授 (30322085) ; 野川 雅道 公立小松大学, 保健医療学部, 准教授 (40292445)
• Project Period (FY): 2014-04-01 – 2019-03-31
• Keywords: 尿成分計測 / 近赤外分光法 / 在宅ヘルスケア / 糖尿病 / 近赤外LED / 試薬レス

Outline of Final Research Achievements

The final goal of this project is to realize a low-price optical and reagent-less system for monitoring multiple components of urine. For this purpose, we examined the absorption spectrum of urine in the near-infrared region and selected 10 wavelength based on the concentration dependence of the spectra including "negative absorption" region. These wavelength were also selected by availability in the market as near-infrared LED (1400-2300nm, 100nm step). Using 51 urine samples obtained from diabetics, multiple regression analysis using all combinations of 10 wavelengths was performed. The optimum number of wavelength and their combination for estimating concentration of glucose, urea, NaCl and creatinine were determined by the value of standard error of prediction. The number of wavelengths required for estimating concentration of urine glucose, urea, NaCl and creatinine were 6, 6, 7 and 6, respectively, suggesting possibility of realizing a low-cost optical urine monitoring system.

Free Research Field

生体医工学

Academic Significance and Societal Importance of the Research Achievements

本研究により、６～７種類の波長の近赤外光を用いて、尿中のグルコース、尿素、NaClおよびクレアチニンの濃度を推定可能な最適な波長組合せを決めることが出来た。これにより近赤外LEDを光源とした簡易な光学式尿成分計測システム具現化の端緒が開かれ、生活習慣病予防のためのホームヘルスケアシステムとしての応用が期待される。さらに、得られたデータから一日のタンパク摂取量の指標となる尿素/クレアチニン比や、一日の塩分摂取量の指標となるNaCl/クレアチニン比が算出可能で、近年普及の著しい臨床現場即時検査（POCT）用の装置としての適用も期待できる。