Sophistication of local gene expression microscopy by adaptive optics

2016 Fiscal Year Final Research Report

• Project/Area Number: 26282128
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: National Institute for Basic Biology
• Principal Investigator: KAMEI Yasuhiro 基礎生物学研究所, 生物機能解析センター, 特任准教授 (70372563)
• Co-Investigator(Renkei-kenkyūsha): TAMADA Yosuke 基礎生物学研究所, 生物進化研究部門, 助教 (50579290) ; HATTORI Masayuki 基礎生物学研究所, 生物機能解析センター, 研究員 (20308208) ; HAYANO Yutaka 国立天文台, 先端技術センター, 准教授 (80390623) ; OYA Shin 国立天文台, TMT推進室, 特任准教授 (80399287)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 顕微鏡 / 赤外レーザー / 熱ショック / 補償光学 / 遺伝子発現 / 遺伝子機能解析 / 個体 / 温度

Outline of Final Research Achievements

Current concept for development of biological microscopy supposes ideal sample that constitutes with homogeneous media for optics. A real living tissue, however, is constituted with heterogeneous media that have different refractive index for optics and they disturb wave front, and finally, an image in deeper area becomes blur. The current concept could not avoid this disturbance. Therefore, new concept is required for biological microscopy. In this project, we apply adaptive optics into microscopy to improve image blurring in deeper area in living tissues. And then, we also apply this technology to new microscope method that enables local gene expression by using heat shock response, called IR-LEGO. Temperature sensing method in living organisms is also required because the IR-LEGO uses invisible infrared light. It means improvement should be evaluated by 3D temperature mapping.

Free Research Field

イメージング、分子遺伝学