Construction of cell-based composite tissue by using an oxygen permeable culture device

2016 Fiscal Year Final Research Report

• Project/Area Number: 26282133
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tohoku University
• Principal Investigator: Anada Takahisa 東北大学, 歯学研究科(研究院), 准教授 (30398466)
• Co-Investigator(Kenkyū-buntansha): 鈴木 治 東北大学, 歯学研究科(研究院), 教授 (60374948)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 細胞・組織工学材料 / 細胞培養デバイス

Outline of Final Research Achievements

Engineering three-dimensional (3D) cellular constructs outside of the body is one of the most important issues for tissue engineering. Increase in size of cellular aggregates causes a depletion of oxygen in the core of aggregates. We have developed an oxygen-permeable cell culture device to prevent hypoxia in the core of the cellular aggregates. The aim of this study was construction of 3D cellular structure consisted of mesenchymal stem cells (MSCs) and vascular endothelial cells based on the oxygen-permeable device we developed. We found that the device was able to improve osteoblastic differentiation of MSCs and generate cellular aggregates consisted of two types of cells. These results suggest that the device is useful for engineering 3D cellular constructs for tissue engineering.

Free Research Field

再生医工学