Development of multi-sensing mass-sensor for quality evaluation of in-vitro fertilized egg

2017 Fiscal Year Final Research Report

• Project/Area Number: 26282143
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Medical systems
• Research Institution: Gunma University
• Principal Investigator: SONE Hayato 群馬大学, 大学院理工学府, 教授 (80344927)
• Co-Investigator(Kenkyū-buntansha): 保坂 純男 群馬大学, 大学院理工学府, 特任教授 (10334129) ; 坂田 利弥 東京大学, 大学院工学系研究科(工学部), 准教授 (70399400)
• Research Collaborator: SAITO Akiko 東京大学, 大学院工学系研究科, 学術支援員
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: カンチレバ－半導体複合型バイオセンサ / 受精卵質量測定 / ピエゾ抵抗カンチレバ / Siナノワイヤバイオセンサ / 検査・診断システム / モニタリング

Outline of Final Research Achievements

In this research, a multi-sensing mass-sensor using piezoresistive cantilever with semiconductor sensor was investigated to measure the mass and respiration activity of a fertilized egg for non-invasive quality evaluation of in-vitro fertilized egg. We fabricated a holder-type piezoresistive cantilever using focused ion beam processing to place a single fertilized egg on the cantilever. Then, we measured a single mouse fertilized egg and sea urchin egg mass by a resonance frequency detection method and deflection detection method using the holder-type cantilever. To verify the fertilized egg mass, we measured the mass and density of a mouse fertilized egg and non-fertilized egg using a sedimentation measurement method. We also developed a Si nanowire sensor, and we demonstrated to detect a low-concentration biomolecules for respiration activity measurement of the fertilized egg.

Free Research Field

ナノメートル計測加工