Exercise related mechanosignling based on nervous system and changes in membrane potential

2016 Fiscal Year Final Research Report

• Project/Area Number: 26282182
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Sports science
• Research Institution: Osaka Dental University
• Principal Investigator: NOTOMI Takuya 大阪歯科大学, 歯学部, 講師 (70542249)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 骨代謝 / 細胞膜電位 / イオンチャネル / 骨芽細胞 / 破骨細胞 / 光遺伝学

Outline of Final Research Achievements

Age-related bone diseases such as osteoporosis are serious problem in an aged society. Mechanical stress and resistance exercise is useful for preventing bone loss. To investigate the relationships between mechanical stress, nervous system and the changes in membrane potential, we overexpressed light-sensitive ion channel and pump in osteoblasts and osteoclasts and identified the targeting molecules via molecular screening based on membrane potential. In invivo experiments, invivo transfection for light-sensitive molecules and cell transplantation were used for investigating bone remodeling. In summary, our results indicate that changes in membrane potential and the receptor for neuronal transmitter affect bone mehanosignaling.

Free Research Field

骨生物学