The role of macrophage circadian clock gene in anti-inflammatory effects of exercise

2016 Fiscal Year Final Research Report

• Project/Area Number: 26282186
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Sports science
• Research Institution: Kyorin University
• Principal Investigator: Kizaki Takako 杏林大学, 医学部, 教授 (00322446)
• Co-Investigator(Renkei-kenkyūsha): OHNO Hideki 杏林大学, 医学部, 名誉教授 (00133819) ; SAKURAI Takuya 杏林大学, 医学部, 講師 (20353477) ; OGASAWARA Junetsu 旭川医科大学, 医学部, 講師 (20415110) ; SHIRATO Ken 杏林大学, 医学部, 助教 (60559384)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: マクロファージ / 運動 / 炎症反応 / 老化 / 時計遺伝子

Outline of Final Research Achievements

Hyperglycemia is associated with chronic low-grade systemic inflammation. In this study, we examined the effects of high-glucose on the regulation of inflammation by the circadian clock gene, Rev-erbα.
High-glucose-cultured macrophages showed an increased expression of phosphorylated Rev-erbα in the nucleus as compared low glucose-cultured cell, although such differences were not observed at the mRNA level. O-linked N-acetylglucosamine (O-GlcNAc) transferase knockdown abolished the high glucose-induced reduction of proteasome activity as well as nuclear accumulation of Rev-erbα. In addition, Rev-erbαknockdown significantly recovered the attenuation of lipopolysaccharide-stimulated production of IL-10 in response to high-glucose culture. These findings suggest that high-glucose condition induces the nuclear accumulation of Rev-erbα via reducing proteasome activity, resulting in the increased inflammation by the suppression of IL-10 expression.

Free Research Field

複合領域