2016 Fiscal Year Final Research Report
Development of Optochemical Biology for photo-mediated regulation of gene function
| Project/Area Number |
26282219
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Basic / Social brain science
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| Research Institution | Toho University |
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Principal Investigator |
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| Research Collaborator |
SUZUKI Akinobu
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ケージド化合物 / 光化学 / 遺伝子 / 有機合成化学 |
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| Outline of Final Research Achievements |
Photochemical activation of caged oligonucleotides enable the regulation of genes of interest within a single-cell level of spatial resolution. In the course of our study of the development of caged oligonucleotides, we designed and synthesized new precursor molecules of photo-releasable nucleotides having an affinity tag for purification and detection. A precursor molecule, Bio-Bhc-diazo comprises three components: biotin for an affinity tag, photoactive Bhc group, and phosphate reactive diazomethyl moiety. We developed a method to modify arbitrary sequence regions of long-chain double-stranded DNA and plasmid DNA with photolabile protecting groups by chemo-enzymatic reactions. We demonstrated that a caged compound of RNA can be synthesized using a novel nucleotide caging reagent having a biotin tag. We demonstrated that this technique can be applied to the gRNA of CRISPR / Cas9 system to synthesize caged gRNA protecting target DNA recognition site or Cas9 binding site.
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| Free Research Field |
生体関連化学
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