Dynamics analysis of biomokecules using fluorescence diamond nanoparticles

2016 Fiscal Year Final Research Report

• Project/Area Number: 26286028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Nanobioscience
• Research Institution: Osaka University (2016); Kyoto University (2014-2015)
• Principal Investigator: Harada Yoshie 大阪大学, 蛋白質研究所, 教授 (10202269)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ナノバイオ / 生物物理学

Outline of Final Research Achievements

We have developed a device and computer programs to measure and analyze ODMR spectra of NV centers in diamond based on an inverted fluorescence microscope, and succeeded in determining the precise orientation of each diamond nanoparticle by applying external magnetic fields sequentially in four inequivalent directions. Diamond nanoparticles were specifically labeled on the EGF receptor via anti-EGF receptor antibody in the cell membrane of A431 cells. By measuring the ODMR spectrum every few minutes and determining the orientation of the diamond nanoparticles, it has become possible to detect the movement of the EGF receptor, that is, the fluctuation motion of the cell membrane. From now on, we will develop this technology to measure various biomolecular dynamics.

Free Research Field

生物物理学