Development of surface selective high-sensitivity fluorescent probe apparatus for microdroplets and its application to biomolecules

2017 Fiscal Year Final Research Report

• Project/Area Number: 26288010
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Kyushu University
• Principal Investigator: Sekiya Hiroshi 九州大学, 理学研究院, 名誉教授 (90154658)
• Co-Investigator(Kenkyū-buntansha): 迫田 憲治 大阪市立大学, 大学院理学研究科, 准教授 (80346767)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: microdroplet / ion trap / energy transfer

Outline of Final Research Achievements

In this research, we developed an experimental apparatus to selectively probe the surface of a single microdroplet levitated in space, and attempted to observe fluorescence excitation energy transfer occurred at the surface. First, a parallel plate ion trap was fabricated to stably levitate a single microdroplet. Microdroplets were generated using an electrospray ionization. Lasing signals from the microdroplet was observed when the droplet in which dye molecules were dissolved were irradiated with a nanosecond pulsed laser. We succeeded in observing the fluorescence excitation energy transfer between the monolayer formed on the surface of the droplet and the dissolved molecule with high sensitivity.

Free Research Field

Physical chemistry