Development of a method for fusion of biological cells onto supported lipid bilayers and its application to membrane protein analyses

2016 Fiscal Year Final Research Report

• Project/Area Number: 26288067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Okayama University
• Principal Investigator: KANETA Takashi 岡山大学, 自然科学研究科, 教授 (20243909)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Keiko 九州大学, 大学院工学研究院, 教授 (30311525) ; TAKEYASU Nobuyuki 岡山大学, 大学院自然科学研究科, 准教授 (90373323)
• Research Collaborator: HIGASHIDANI Naoki ; KANAJI Keisuke ; KOBAYASHI Sakurako ; MAKI Tomomi ; OGAWA Kazuma ; KARITA Shingo ; MITSUNOBU Manami ; SHIMADA Yuhi ; HARADA Airi ; KUDO Simire ; ISOYAMA Mika ; KUBOI Mai ; FUJII Tatsuya ; MIKI Shoko
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 膜タンパク質 / 単一細胞計測 / 細胞融合 / 電気泳動 / 単一分子検出 / マイクロ流体デバイス

Outline of Final Research Achievements

The present study aimed the development of the method to incorporate membrane proteins in real biological cells into supported lipid bilayers and its application to the separation and determination of the membrane proteins. We constructed a system for observing total internal reflection fluorescence. We fabricated the supported lipid bilayers and incorporated biological cells into the supported lipid bilayers in order to develop the analytical method of the membrane proteins localized in biological cell membranes.

Free Research Field

分析化学