Highly-sensitive Immunosensing Based on Signal Amplification Systems by Functional Particles

2016 Fiscal Year Final Research Report

• Project/Area Number: 26288071
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: University of Hyogo
• Principal Investigator: Mizutani Fumio 兵庫県立大学, 物質理学研究科, 教授 (80118603)
• Co-Investigator(Kenkyū-buntansha): 安川 智之 兵庫県立大学, 物質理学研究科, 准教授 (40361167)
• Co-Investigator(Renkei-kenkyūsha): MATSUE Tomokazu 東北大学, 原子分子材料科学高等研究機構, 教授 (70173797)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ゲル粒子 / 高感度免疫アッセイ / 酵素 / 電解酸化 / 誘電泳動

Outline of Final Research Achievements

The rapid, simple and highly-sensitive immunosensing have been developed. The recognition behavior of target molecules by the antibody was labeled with a large number of enzymes. The particles of alginate gel with glucose oxidase were fabricated by the electrochemical oxidation of metallic cupper to cupper ions in the microwell array electrodes. The enzyme activity enclosed in particles were estimated by the electrochemical microscopy. The products generated by the enzyme reaction were sensitively detected by the chemical amplification system including the redox cycling of electroactive species and accumulation of the oxidation reaction of electroactive species. The amplification system can be applied to measure marker protein. The gel particles fabricated was captured at the tip of microelectrode by using the dielectrophoresis. The gel particles with the antibody was captured at the tip modified with the antibody in the presence of antigen.

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