Non-invasive chemical diagnostic methods by flow breath analysis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26288072
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Aichi Institute of Technology
• Principal Investigator: Teshima Norio 愛知工業大学, 工学部, 教授 (30292501)
• Co-Investigator(Kenkyū-buntansha): 梅村 知也 東京薬科大学, 生命科学部, 教授 (10312901) ; 澤野 誠 埼玉医科大学, 医学部, 准教授 (40242143) ; 村上 博哉 愛知工業大学, 工学部, 講師 (40515128)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 非侵襲分析 / 呼気分析 / 流れ分析 / 呼気アセトン / 吸光光度法 / HPLC

Outline of Final Research Achievements

We have proved so far that flow injection analysis (FIA) and related techniques are useful for human breath analysis. In this project, a flow injection fluorometric determination of acetone in human breath was developed. Gaseous acetone in breath was sampled with a porous membrane based diffusion scrubber, then the absorbing solution including acetone merged with an alkaline salicylaldehyde, and the fluorescence intensity of salicylaldehyde decreased with increasing the acetone concentration. On the other hand, SIEMA systems we conceived can minimize the amount of sample solution and the reagent consumption. Also, it is a useful concept for automatic solution handling. We also proposed a SIEMA system for automatic pre-column derivatization HPLC determination of formaldehyde, acetaldehyde, acrolein and acetone. In the near future, an automated pre-column derivatization HPLC method for the determination of aldehydes and acetone in human breath using a SIEMA system would be developed.

Free Research Field

分析化学