Chemically modified DNAs cotrolled by triplex forming oligodeoxynucleotides

2016 Fiscal Year Final Research Report

• Project/Area Number: 26288075
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Seio Kohji 東京工業大学, 生命理工学院, 准教授 (20313356)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 三重鎖DNA / 人工遺伝子 / 蛍光核酸

Outline of Final Research Achievements

We developed artificial DNAs whose function could be controlled by triplex formation. First we developed artificial genes incorporating pseudouracil and pseudoisocytosine which had hydrogen bond donor in their minor groove sides. The modified DNAs can be transcribed by T7-RNA polymerase and the transcrption was inhibited by the addition of triplex forming oligonucleotides incorporating artificial bases which can form hydrogen bond with pseudouracil or pseudoisocytosine. We also developed fluorescent DNAs having aromatic groups in their minor groove. The spectroscopic analyses revealed that the fluorescence of the DNAs can be enhanced by addition of triplex forming oligonucleotides.

Free Research Field

核酸有機化学