2016 Fiscal Year Final Research Report
Evaluation of oxygen effect on radiolysis of tumor tissue on the basis of quantitative analysis of oxygen concentration
| Project/Area Number |
26288078
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Aoyama Gakuin University (2015-2016)
Kyoto University (2014) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
孫 安生 京都大学, 工学(系)研究科(研究院), 研究員 (30447924)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 放射線 / がん治療 / 酸素濃度計測 / 分子プローブ |
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| Outline of Final Research Achievements |
Understanding tumor microenvironment is crucial for analyzing response of solid tumor to therapy and in the augmentation of that response. Oxygen status, which fluctuates quickly, is one of the most important components that should be analyzed, because oxygen makes a profound impact on treatment resistance, especially against radiotherapy. Therefore, tractable technology for the oxygen status has been imperative.
We successfully monitored oxygen fluctuation in cell nucleus and tumor tissue directly. Ruthenium complex with Hoechst unit (Ru-Hoechst) and ruthenium complex-labeled oligonucleotides (DRC) with pyrene and nitroimidazole units were synthesized and we evaluated their properties. These probe showed oxygen-dependent phosphorescence at cellular nucleus and tumor tissue and therefore, we could monitor the oxygen levels. These probes will be applicable to monitoring of oxygen levels and assessment of response of tumor tissue against radiation on the basis of oxygen quantification.
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| Free Research Field |
生体分析化学
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