Development of supramolecular construction techniques of proteins and their functionalization using domain swapping

2016 Fiscal Year Final Research Report

• Project/Area Number: 26288080
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: HIROTA Shun 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (90283457)
• Co-Investigator(Renkei-kenkyūsha): HIGUCHI Yoshiki 兵庫県立大学, 大学院生命理学研究科, 教授 (90183574) ; NAGAO Satoshi 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (30452535) ; YAMANAKA Masaru 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (60632825)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: タンパク質 / 生体分子 / ナノバイオ / 超分子化学 / 多量体 / 立体構造 / ドメインスワッピング

Outline of Final Research Achievements

A certain amount of molten globule state oligomers of horse cyt c converted to domain-swapped oligomers by folding from the acid molten globule state to neutral pH state under high protein concentrations. Domain-swapped dimer stability of horse cyt c was less affected by the Met80 coordination to the heme. Two domain-swapped structures of thermostable cyt c552 were detected for its hinge loop mutant. Domain swapping was shown to also occur for thermostable cyt c552 when expressed in E. coli cells. A tetramer of cyt c′ was constructed with one domain-swapped dimer and two monomers. An artificial myoglobin heterodimer with two different heme active sites was constructed based on domain swapping.

Free Research Field

タンパク質科学