2016 Fiscal Year Final Research Report
Investigation of microbial polymer-degrading enzymes and their mechanism
| Project/Area Number |
26288085
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Green/Environmental chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Research Collaborator |
NUMBI Ramudu Kamini Central Leather Research Institute, Department of Biotechnology, 部門長
SHAH Aamer Ari Quaid-i-Azam University, Department of Microbiology, 准教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 生分解性物質 / 微生物酵素 |
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| Outline of Final Research Achievements |
In this study, genetic structure and substrate specificity of biodegradable plastic-degrading enzymes derived from microorganisms and their industrial application were investigated. First, the whole genome analysis of Roseateles depolymerans TB-87 was performed to identify its degradation enzyme genes and analyzed the homology with the enzyme derived from TB-71. Furthermore, functional analysis of chaperone-like protein and related enzymes of other plastic-degrading bacteria were carried out to clarify the relationship between amino acid sequence and substrate specificity. Furthermore, culture conditions and medium cost were examined for high expression of these microbial enzymes.
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| Free Research Field |
産業微生物資源学
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