2016 Fiscal Year Final Research Report
Application of minute biofluid handling techniques using an artificial cell membrane
| Project/Area Number |
26289038
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Fluid engineering
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| Research Institution | Chuo University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OKANO Taiji 中央大学, 理工学部, 助教 (60622082)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | バイオリアクター / リポソーム / 膜融合 / 膜分裂 / ゲノム増幅 / PCR |
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| Outline of Final Research Achievements |
We aimed to establish an ultra-small biochemical assay platform using giant liposomes as a femtoliter-volume reactor, which enables addition, mixing, and aliquoting. As a means of GUV fusion, we studied PEG-mediated fusion method in addition to the well-established GUV electrofusion method, and found that PEG-fusion is more suitable for fusing GUV and the live cell membrane. For aliquoting, we thoroughly studied the effect of depletion-volume effect and addition of mono-acyl lipids. Finally we demonstrated the three distinct biochemical reactions in GUVs; (1) an enzyme reaction, (2) the whole-genome amplification, and (3) the reverse transcription and polymerase chain reaction (RT-PCR) to show that the sequential handling of these reagents is possible.
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| Free Research Field |
バイオ流体力学
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