Development of methods for microbial source tracking in drinking water sources using host-specific viral markers

2016 Fiscal Year Final Research Report

• Project/Area Number: 26289182
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: University of Yamanashi
• Principal Investigator: HARAMOTO Eiji 山梨大学, 総合研究部, 准教授 (00401141)
• Co-Investigator(Kenkyū-buntansha): 岸田 直裕 国立保健医療科学院, 生活環境研究部, 主任研究官 (10533359) ; 端 昭彦 京都大学, 工学研究科, 日本学術振興会特別研究員 (70726306)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ウイルス / 濃縮法

Outline of Final Research Achievements

This study aimed to develop methods for concentrating viruses in drinking water source and drinking water, mainly focusing a NanoCeram electropositive filter. Recovery test of F-specific RNA coliphage Qbeta and ColorSeed, a commercially available product containing Cryptosporidium and Giardia (oo)cysts, was conducted to develop a method for simultaneous concentration of viruses and protozoa in a single water sample. Furthermore, recovery of F-specific RNA coliphage MS2 and pepper mild mottle virus, a plant virus, using the NanoCeram electropositive filter was tested as well as methods using electronegative membrane filters. This study has successfully developed novel virus concentration methods that can be used for microbial source tracking using viruses.

Free Research Field

水環境工学