Development of the innovative process for material production using functional diatoms based on the biosilica engineering

2016 Fiscal Year Final Research Report

• Project/Area Number: 26289309
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Tanaka Tsuyoshi 東京農工大学, 工学(系)研究科(研究院), 教授 (20345333)
• Co-Investigator(Kenkyū-buntansha): 吉野 知子 東京農工大学, 工学(系)研究科(研究院), 准教授 (30409750) ; 真山 茂樹 東京学芸大学, 教育学部, 教授 (40199914)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 有用珪藻 / バイオシリカ工学 / 自己凝集 / 有用物質生産

Outline of Final Research Achievements

This study aimed to develop the innovative process for material production using functional diatoms based on the biosilica engineering, in which biosilica cell wall of diatoms were engineered via genetic manipulation. First, genetic manipulation technique for stable cell-surface display of recombinant proteins on the frustules was established in the pennate diatom, Fistulifera solaris. Subsequently, cell aggregation-inducible functional peptides were displayed on the surface of the cell walls (frustules), and the peptide-mediated cell harvesting was demonstrated. Gene knockdown technique was also established in this study in order to efficiently disrupt the cells for improved material extraction. Furthermore, structures and chemical compositions analyses for organic matrix associated with biosilica cell walls were carried out. These outcomes could pave the way to develop the novel bioprocesses for efficient material production in diatoms

Free Research Field

生物機能・バイオプロセス