Analysis,design and control of 2D and 3D dynamics of cell mimetic membrane

2016 Fiscal Year Final Research Report

• Project/Area Number: 26289311
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: Takagi Masahiro 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (00183434)
• Co-Investigator(Renkei-kenkyūsha): SHIMOKAWA Naofumi 北陸先端科学技術大学院大学, 先端科学技術研究科, 助教 (20700181)
• Research Collaborator: HAMADA Tsutomu 北陸先端科学技術大学院大学, 先端科学技術研究科, 准教授 (40432140)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: リポソーム / ダイナミクス / ラフト / アミロイド / DNA / 生理活性物質 / 生物模倣

Outline of Final Research Achievements

With respect to cell signaling, it is pointed out that not only membrane receptors and channels but also dynamic structural changes of membranes are important. We are conducting research by classifying membrane dynamics into two-dimensional dynamics (phase separation) and three dimensional dynamics (morphological change including vesicle formation). Here we describe the interaction between DNA and membrane and the interaction between amyloid and membrane. About DNA, there is clear difference between coil state and globule state in the sites of interaction with the phase separated structure of the membrane. Regarding amyloid β, the model membrane revealed that 7-ketocholesterol, a type of oxidized cholesterol, is involved in membrane binding, and the results were also confirmed in living cells.

Free Research Field

生物機能工学