Improvement of infulenza vaccine production by transgenic chicken

2016 Fiscal Year Final Research Report

• Project/Area Number: 26289312
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Nagoya University
• Principal Investigator: Shinji Iijima 名古屋大学, 工学(系)研究科(研究院), 教授 (00168056)
• Co-Investigator(Kenkyū-buntansha): 小野 悦郎 九州大学, 医学(系)研究科(研究院), 教授 (00160903)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: トランスジェニックニワトリ / インフルエンザ / シアル酸

Outline of Final Research Achievements

In order to produce influenza vaccine effectively, we tried to establish transgenic chicken that lays eggs suitable for virus propagation. Chimeric chickens were established those expressed either phiC31 recombinase or sialic acid transferase by using retrovirus and lentivirus vectors. By crossing these chimeric chicken, we tried to establish transgenic chickens. Although we have analyzed more than 250 offspring, we could not detect transgenic chicken. The expression of IFITMs those prevent influenza virus propagation, was analyzed in chicken, and certain level of expression could be detected in chorioallantoic membrane where influenza virus propagates. We also establish in vitro and viral particle free assay system of influenza virus propagation.

Free Research Field

生物生体工学