Manipulation technology development of chicken pluripotent stem cells for constructing transgenic bioreactors

2016 Fiscal Year Final Research Report

• Project/Area Number: 26289316
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyushu University
• Principal Investigator: Kamihira Masamichi 九州大学, 工学(系)研究科(研究院), 教授 (40202022)
• Co-Investigator(Renkei-kenkyūsha): KAWABE Yoshinori 九州大学, 工学研究院, 助教 (30448401)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: バイオテクノロジー / 生物・生体工学 / 生体バイオリアクター / ニワトリ / 多能性幹細胞

Outline of Final Research Achievements

This study aimed to develop a technology to generate transgenic chickens derived from pluripotent stem cells as a new production platform for biopharmaceuticals. Total RNAs extracted from chicken blastodermal cells, containing pluripotent stem cells, were applied for the massive sequencing technology to analyze expression profiles of reprogramming factor genes. Using reprogramming factor genes identified by the transcriptome analysis, chicken induced pluripotent stem cells were generated. Furthermore, a genome editing technique, CRISPR/Cas9 system was applied for targeting a sex determination gene, DMRT1. A targeting vector designed efficiently altered the target region in DMRT1 gene of chicken cells.

Free Research Field

生物工学