2016 Fiscal Year Final Research Report
Generations of mice for imaging of cardiovascular system
| Project/Area Number |
26290030
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
Ema Masatsugu 滋賀医科大学, 動物生命科学研究センター, 教授 (60359578)
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| Co-Investigator(Kenkyū-buntansha) |
三輪 佳宏 筑波大学, 医学医療系, 講師 (70263845)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 血管内皮細胞 / 腫瘍 / 発生 |
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| Outline of Final Research Achievements |
We generated a novel transgenic (Tg) mouse that expresses a bioluminescent reporter protein, Nano-lantern, under the control of Fetal liver kinase 1 (Flk1). Flk1-Nano-lantern BAC Tg mice recapitulated endogenous Flk1 expression in endothelial cells and lymphatic endothelial cells during development and tumour growth. Importantly, bioluminescence imaging of endothelial cells from the aortic rings of Flk1-Nano-lantern BAC Tg mice enabled us to observe endothelial sprouting for 18 hr without any detectable phototoxicity. Furthermore, Flk1-Nano-lantern BAC Tg mice achieved time-lapse luminescence imaging of tumour angiogenesis in freely moving mice with implanted tumours.
We also Lrrc33 KO mice and demonstrated that Lrrc33 is important for the function of tumour endothelial cells.
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| Free Research Field |
発生工学
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