Development of genome editing technologies in laboratory rats

2017 Fiscal Year Final Research Report

• Project/Area Number: 26290033
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Osaka University (2015-2017); Kyoto University (2014)
• Principal Investigator: Mashimo Tomoji 大阪大学, 医学系研究科, 准教授 (80397554)
• Co-Investigator(Kenkyū-buntansha): 金子 武人 岩手大学, 理工学部, 准教授 (30332878) ; 夘野 善弘 大阪大学, 医学系研究科, 助手 (80252683)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: ゲノム編集 / ラット / 遺伝子 / 脳神経疾患 / 実験動物

Outline of Final Research Achievements

In order to develop novel genome editing technologies in laboratory rats, we succeeded to generate genome-edited rats, such as knockouts, SNP exchange, a dozen bp insertion, and several Kbp deletion, using the CRISPR/Cas9 system. We also developed a GFP cassette knock-ins using long single-stranded DNA (lssDNA), a CLICK method to efficiently generate conditional knockout rats, a 2H2OP method to knock-in large scale genomic regions such as 200 Kb BAC. The 2H2OP method and the CLICK method were patented. We are developing in vivo genomic editing technology using Cas9-transgenic rats, as well as are generating new rat models of Parkinson's disease using the rat genome editing technology we established in this study.

Free Research Field

ゲノム編集