Induction of human allergic responses in 2nd generation NOG mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 26290034
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Central Institute for Experimental Animals
• Principal Investigator: TAKAHASHI TAKESHI 公益財団法人実験動物中央研究所, その他部局等, 室長 (80335215)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ヒト化マウス

Outline of Final Research Achievements

We extensively gene-modified immunodeficient NOG mice and 15 different sub-strains (2nd generation NOG mice) were generated. The hematopoiesis and immune functions were investigated after transplantation of human hematopoietic stem cells. For example, NOG-pRORgt-gc transgenic (Tg) mice showed enhanced human IgG production. NOG-human (h)TLSP-IL-13 Tg showed human IgE production. And 3 different NOG-HLA-A Tg and 2 different NOG-HLA-DR Tg mice were generated. We also demonstrated that human food allergy symptoms could be recapitulated in humanized NOG-hGM-CSF/IL-3 Tg mice with multiple lineages of human hematopoietic cells. Lactoglobulin specific IgE recombinant antibody was given to the humanized NOG-hGM-CSF/IL-3 and subsequently they were fed with Lactoglobulin proteins. The mice showed anaphylaxis manifested by the reduction of body temperature.

Free Research Field

免疫学