Mechanism of the nuclear retention of unspoiled RNAs that ensures proper expression of genomic information

2016 Fiscal Year Final Research Report

• Project/Area Number: 26290062
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Kobe University
• Principal Investigator: Sakamoto Hiroshi 神戸大学, 理学(系)研究科(研究院), 教授 (00187048)
• Co-Investigator(Kenkyū-buntansha): 井上 邦夫 神戸大学, 理学(系)研究科(研究院), 教授 (40252415) ; 鈴木 穣 東京大学, 新領域創成科学研究科, 教授 (40323646) ; 中井 謙太 東京大学, 医科学研究所, 教授 (60217643)
• Research Collaborator: FUKUMOTO Shoichi ; AIZAWA Risuke ; MIWA Takashi ; TAKASAKI Teruaki
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ゲノム機能発現 / 遺伝子発現調節 / RNAスプライシング / 核外輸送 / 品質管理 / 翻訳制御

Outline of Final Research Achievements

NXF-1 and CRM1 are well conserved among eukaryotes and act as nuclear export receptors for mRNAs and snRNAs, respectively. EJC is a complex formed just upstream of exon-exon junctions after pre-mRNA splicing and has various functions in RNA metabolism. Depletion of Y14, a core component of EJC, in the Nematode C. elegans, results in the cytoplasmic leakage of unspliced RNAs, which depends on CRM1 and NXF-2, an NXF-1-like protein. Analysis of C. elegans and cultured cells expressing epitope-tagged NXF-2 showed that NXF-2 interacts with both NXT-1, a nuclear export factor, and eIF4E, a translation initiation factor, and functions as a translation activator when tethered on mRNA.

Free Research Field

分子生物学