In vitro reconstitution of the initiation process of transcription-coupled nucleotide-excision repair

2017 Fiscal Year Final Research Report

• Project/Area Number: 26291005
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Nagoya University (2015-2017); Nagasaki University (2014)
• Principal Investigator: OGI Tomoo 名古屋大学, 環境医学研究所, 教授 (80508317)
• Co-Investigator(Kenkyū-buntansha): 真下 知士 大阪大学, 医学系研究科, 准教授 (80397554)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: DNA修復 / 転写と共役したヌクレオチド除去修復

Outline of Final Research Achievements

Transcription-coupled nucleotide-excision repair (TC-NER) is one of the versatile DNA repair process that removes major UV-induced DNA lesions from actively transcribed genes. To investigate the initiation process of TC-NER reaction, we focused on a molecular mechanism that involves DNA-damage induced ubiquitination of RNA polymerase IIo (RNA pol IIo). The process is dependent on a recently identified TC-NER factor, UVSSA.
To determine the ubiquitination sites, we performed in vitro ubiquitination assay as well as SILAC-based High-Resolution Accurate Mass (HRAM) spectrometry. From the studies, we identified UVSSA-dependent ubiquitination sites required for the efficient TC-NER activity.

Free Research Field

DNA修復学、分子生物学