Study on the mitochondria-sarcoplasmic reticulum 3D Ca crosstalk in cardiomyocytes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26291019
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: University of Fukui
• Principal Investigator: Takeuchi Ayako 福井大学, 学術研究院医学系部門, 特命助教 (00378704)
• Co-Investigator(Kenkyū-buntansha): 天野 晃 立命館大学, 生命科学部, 教授 (60252491)
• Research Collaborator: IINO Satoshi 福井大学, 学術研究院医学系部門, 教授 ; HORIGUCHI Kazuhide 福井大学, 学術研究院医学系部門, 准教授 ; FUKAZAWA Yugo 福井大学, 学術研究院医学系部門, 教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ミトコンドリア / 筋小胞体 / 心室筋細胞 / 心房筋細胞

Outline of Final Research Achievements

The purpose of the study was to clarify physiological roles of mitochondria-sarcoplasmic reticulum 3D Ca crosstalk in cardiomyocytes. From combination study of experiments and mathematical simulations, we obtained following results. 1. Parameters explaining mitochondria-sarcoplasmic reticulum localization were obtained by analyzing electron microscopy data on mouse cardiomyocytes. 2. In vitro localization analyses were performed using HEK293 cells and mouse cardiomyocytes and it was suggested that mitochondrial Na/Ca exchanger NCLX and sarcoplasmic reticulum Ca pump SERCA were localized in close proximity to each other. 3. A mathematical model of a detailed mitochondrial oxidative phosphorylation was newly constructed. The model predicted the contribution of Ca-dependent regulations of mitochondrial energy metabolism with various compositions of energy substrates. In addition, the roles of mitochondria-sarcoplasmic reticulum crosstalk were also analyzed.

Free Research Field

細胞生理学、フィジオーム