Spatio-temporal analysis of extracellular ATP signaling of pancreatic islets

2016 Fiscal Year Final Research Report

• Project/Area Number: 26291027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Kyoto University
• Principal Investigator: Imamura Hiromi 京都大学, 生命科学研究科, 准教授 (20422545)
• Co-Investigator(Renkei-kenkyūsha): FUJIWARA Takahiro 京都大学, 物質-細胞統合システム拠点, 講師 (80423060)
• Research Collaborator: YOSHIDA Tomoki 京都大学, 大学院生命科学研究科, 研究員 ; IWAKIRI Ryuta 京都大学, 大学院生命科学研究科, 大学院生 ; NISHIYAMA Sui 京都大学, 大学院生命科学研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ATP / バイオセンサー / 蛍光タンパク質 / ブリン受容体 / インスリン

Outline of Final Research Achievements

The aim of this work was to establish methods to visualize extracellular ATP, in order to elucidate extracellular ATP signaling inside a live tissue, including a pancreatic islet.
First, we succeeded in imaging extracellular ATP by using a FRET-based biosensor. We also succeeded in developing two new ATP biosensors; single FP-based "QUEEN" and BRET-based "BTeam". Although we could use these new biosensors to monitor intracellular ATP, several problems remained to be solved before applying them to extracellular ATP imaging.

Free Research Field

バイオイメージング