2017 Fiscal Year Final Research Report
Site selective cellular analysis by solid-state NMR
| Project/Area Number |
26291029
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KOJIMA Chojiro 大阪大学, 蛋白質研究所, 准教授 (50333563)
MATSUKI Yoh 大阪大学, 蛋白質研究所, 助教 (70551498)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 核磁気共鳴 / 細胞生物学 / 構造生物学 |
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| Outline of Final Research Achievements |
The thermal equilibrium NMR signal intensities give the quantitative information for all the cellular molecules at low temperatures where molecular motions are suppressed. The molecules for a single cell were counted by combined use of optical and electron microscope analyses, which enabled us to trace the biosynthetic processes by using the 13C labeled media without the purification. Applications of our magic-angle-spinning DNP NMR spectrometer with a closed-cycle He-gas system at low temperature 30 K and 16.4 T are also shown.
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| Free Research Field |
生物物理学
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