The role of RNA-binding protein for long-distance transportation of mRNA within embryonic neural progenitor cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26291046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tohoku University
• Principal Investigator: OSUMI Noriko 東北大学, 医学(系)研究科(研究院), 教授 (00220343)
• Co-Investigator(Kenkyū-buntansha): 稲田 仁 東北大学, 医学(系)研究科(研究院), 講師 (60419893) ; 吉川 貴子 東北大学, 医学(系)研究科(研究院), 助教 (90727851)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 神経発生 / RNA結合タンパク質 / FMRP / 放射状グリア / mRNA輸送

Outline of Final Research Achievements

The mammalian cortex has expanded during evolution and its primordium consists with radial glial (RG) cells as neural progenitors. RG cells have long processes stretching between apical and basal surface of the cortical primordium. We would like to understand molecular mechanisms for long-distance transportation within the RG cell, and focused on an RNA-binding protein, i.e., fragile X mental retardation protein (FMRP) , coded by Fmr1 gene. We performed RIP-ChIP analyses and found candidate mRNAs as targets of FMRP, and examined expression profiles of the candidate genes. Immunohistochemical analyses showed decreased expression of some of the candidate genes at the protein level, suggesting a possibility that these transcripts can be transported by FMRP.

Free Research Field

神経発生