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2017 Fiscal Year Annual Research Report

Investigation of the diversity of visual and non-visual UV reception in jumping spiders

Research Project

Project/Area Number 26291070
Research InstitutionOsaka City University

Principal Investigator

小柳 光正  大阪市立大学, 大学院理学研究科, 准教授 (30379276)

Project Period (FY) 2014-04-01 – 2018-03-31
Keywords生理学 / 光生物学 / 感覚 / ロドプシン
Outline of Annual Research Achievements

多くの動物は、UV光を受容し、視覚や視覚以外の役割(非視覚)に利用している。本研究では、動物のUV光利用の多様性を知る目的で、視覚(主眼)・非視覚(後中眼)に機能分化した2種類の眼でUV光受容を行うハエトリグモに着目し、分子基盤の解析を起点として、それぞれのUV光受容が関わる生理機能の解明を進めている。また、対比として可視光受容についても同様の解析を行い、平成29年度は以下の成果を得た。

・昨年度までに取得した、多種類のハエトリグモおよび他のクモ類(カニグモ、ハナグモ、コモリグモ等)の光受容タンパク質について、培養細胞発現系を用いてリコンビナント光受容タンパク質の取得に成功した。それぞれの光受容タンパク質について、分光学的解析によって吸収スペクトルを明らかにし、クモ類の「光利用」と「系統関係・生態」との連関を明らかにした。
・クモ類において網膜以外の組織で発現する新規非視覚光受容タンパク質が、「暗状態で活性化し、光刺激によって不活性化する」という、従来の光受容タンパク質とは逆の性質を持つことを生化学的解析によって明らかにした。また、他のクモ類についても同光受容タンパク質の解析を行い、吸収スペクトルを明らかにした。複数のクモ類について、この非視覚の光受容タンパク質の吸収スペクトルと視覚の光受容タンパク質の吸収スペクトルを比較した結果、視覚と非視覚では、受容する光の色に対する要求が異なることが明らかになった。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Causes of Carryover

29年度が最終年度であるため、記入しない。

Expenditure Plan for Carryover Budget

29年度が最終年度であるため、記入しない。

  • Research Products

    (34 results)

All 2018 2017 Other

All Int'l Joint Research (4 results) Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (25 results) (of which Int'l Joint Research: 12 results,  Invited: 2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] The University of Manchester(英国)

    • Country Name
      UNITED KINGDOM
    • Counterpart Institution
      The University of Manchester
  • [Int'l Joint Research] Paul Scherrer Institute(スイス)

    • Country Name
      SWITZERLAND
    • Counterpart Institution
      Paul Scherrer Institute
  • [Int'l Joint Research] Institut de la Vision(フランス)

    • Country Name
      FRANCE
    • Counterpart Institution
      Institut de la Vision
  • [Int'l Joint Research] Salk Institute for Biological Studies(米国)

    • Country Name
      U.S.A.
    • Counterpart Institution
      Salk Institute for Biological Studies
  • [Journal Article] An all-trans-retinal-binding opsin peropsin as a potential dark-active and light-inactivated G protein-coupled receptor2018

    • Author(s)
      Nagata Takashi、Koyanagi Mitsumasa、Lucas Robert、Terakita Akihisa
    • Journal Title

      Scientific Reports

      Volume: 8 Pages: 3535

    • DOI

      10.1038/s41598-018-21946-1

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Convergent evolution of tertiary structure in rhodopsin visual proteins from vertebrates and box jellyfish2018

    • Author(s)
      Elliot Gerrard, Eshita Mutt, Takashi Nagata, Mitsumasa Koyanagi, Tilman Flock, Elena Lesca, Gebhard Schertler, Akihisa Terakita, Xavier Deupi, Robert Lucas
    • Journal Title

      Proceedings of the National Academy of Sciences of USA

      Volume: 印刷中 Pages: -

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Expression and light-dependent translocation of β-arrestin in the visual system of the terrestrial slug Limax valentianus2017

    • Author(s)
      Matsuo Ryota、Takatori Yuka、Hamada Shun、Koyanagi Mitsumasa、Matsuo Yuko
    • Journal Title

      The Journal of Experimental Biology

      Volume: 220 Pages: 3301~3314

    • DOI

      10.1242/jeb.162701

    • Peer Reviewed / Open Access
  • [Presentation] Characterization of arrestin binding to parapinopsin, a photopigment involved in pineal wavelength discrimination2018

    • Author(s)
      Takashi Nagata, Mitsumasa Koyanagi, Emi Kawano-Yamashita, Seiji Wada, Akihisa Terakita
    • Organizer
      9th OCARINA International Symposium
    • Int'l Joint Research
  • [Presentation] Color opponency with a single kinds of bistable opsin in the zebrafish pineal organ2018

    • Author(s)
      Seiji Wada, Baoguo Shen, Emi Kawano-Yamashita, Takashi Nagata, Masahiro Hibi, Satoshi Tamotsu, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      9th OCARINA International Symposium
    • Int'l Joint Research
  • [Presentation] Estimating spectral sensitivities of various opsins based on biochemical responses of cultured cells expressing opsins2018

    • Author(s)
      Tomohiro Sugihara, Takashi Nagata, Benjamin Mason, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      9th OCARINA International Symposium
    • Int'l Joint Research
  • [Presentation] Contribution of parapinopsin “bistability” to light-responses in the zebrafish pineal organs2018

    • Author(s)
      Baoguo Shen, Seiji Wada, Emi Kawano-Yamashita, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      9th OCARINA International Symposium
    • Int'l Joint Research
  • [Presentation] Investigation of amino acid residues involved in spectral tuning of butterfly long-wavelength-sensitive opsins2018

    • Author(s)
      Tomoka Saito, Mitsumasa Koyanagi, Tomohiro Sugihara, Kentaro Arikawa, Akihisa Terakita
    • Organizer
      9th OCARINA International Symposium
    • Int'l Joint Research
  • [Presentation] Functional properties of diverse animal rhodopsins and their potentials for optical regulation of cell signaling2017

    • Author(s)
      Mitsumasa Koyanagi
    • Organizer
      International Symposium on Biophysics of Rhodopsins
    • Int'l Joint Research / Invited
  • [Presentation] Mutational analysis of the Schiff base counterion in Jumping spider rhodopsin, an invertebrate Gq-coupled rhodopsin2017

    • Author(s)
      Takashi Nagata, Mitsumasa Koyanagi, Hisao Tsukamoto, Eshita Mutt, Xavier Deupi, Gebhard Schertler, Akihisa Terakita
    • Organizer
      International Symposium on Biophysics of Rhodopsins
    • Int'l Joint Research
  • [Presentation] Amino acid residues involved in Spectral tuning that accounts for difference in absorption maxima between two kinds of butterfly long-wavelength-sensitive opsins2017

    • Author(s)
      Tomoka Saito, Mitsumasa Koyanagi, Tomohiro Sugihara, Kentaro Arikawa, Akihisa Terakita
    • Organizer
      International Symposium on Biophysics of Rhodopsins
    • Int'l Joint Research
  • [Presentation] Investigation of wavelength discrimination involving bistable parapinopsin in the zebrafish pineal organ2017

    • Author(s)
      Seiji Wada, Baoguo Shen, Emi Kawano-Yamashita, Takashi Nagata, Satoshi Tamotsu, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      International Symposium on Biophysics of Rhodopsins
    • Int'l Joint Research
  • [Presentation] ナメクジの網膜におけるβアレスチンの発現と光依存的な細胞内局在変化2017

    • Author(s)
      松尾 亮太,鷹取 由佳,濱田 俊,小柳 光正,松尾 優子
    • Organizer
      日本動物学会九州支部・九州沖縄植物学会・日本生態学会九州地区 2017三学会合同大分大会
  • [Presentation] Optical regulation of cell signaling using bistable animal opsins2017

    • Author(s)
      Mitsumasa Koyanagi, Baoguo Shen, Takashi Nagata, Lanfang Sun, Seiji Wada, Satomi Kamimura, Eriko Kage-Nakadai, Akihisa Terakita
    • Organizer
      2017 FASEB SUMMER RESEARCH CONFERENCES
    • Int'l Joint Research
  • [Presentation] Molecular properties of animal non-visual opsins and their optogenetic potentials2017

    • Author(s)
      Baoguo Shen, Takashi Nagata, Seiji Wada, Satomi Kamimura, Eriko Kage-Nakadai, Robert Lucas, Gebhard Schertler, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      17th HFSP Awardees Meeting
    • Int'l Joint Research
  • [Presentation] 有袋類に着目した哺乳類の進化過程で失われた非視覚系光受容タンパク質の機能解析2017

    • Author(s)
      小柳 光正,冨田 和志,清成 寛,寺北 明久
    • Organizer
      日本進化学会第19回大会
  • [Presentation] Contribution of a molecular property of a pineal opsin parapinopsin to cellular responses2017

    • Author(s)
      Seiji Wada, Baoguo Shen, Emi Kawano-Yamashita, Takashi Nagata, Satoshi Tamotsu, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      第55回生物物理学会年会
  • [Presentation] パラピノプシンの双安定性に着目した細胞応答の波長依存的な光制御2017

    • Author(s)
      小柳 光正,沈 宝國,永田 崇,孫 蘭芳,和田 清二,上村 了美,中台(鹿毛)枝里子,寺北 明久
    • Organizer
      日本動物学会第88回大会
  • [Presentation] 松果体波長識別応答に関わる光受容タンパク質パラピノプシンのアレスチン結合に関する解析2017

    • Author(s)
      永田 崇,小柳 光正,山下(川野)絵美,和田 清二,小澤 岳昌,寺北 明久
    • Organizer
      日本動物学会第88回大会
  • [Presentation] ゼブラフィッシュ松果体の波長識別応答における光受容タンパク質パラピノプシンの分子特性の重要性2017

    • Author(s)
      和田 清二,沈 宝國,山下(川野)絵美,永田 崇,保 智己,小柳 光正,寺北 明久
    • Organizer
      日本動物学会第88回大会
  • [Presentation] ゼブラフィッシュ松果体におけるメラトニン分泌の光制御に関わるオプシンの解析2017

    • Author(s)
      寺北 明久,和田 清二,藤井 耀,小柳 光正
    • Organizer
      日本動物学会第88回大会
  • [Presentation] ゼブラフィッシュ松果体の光応答におけるパラピノプシンの分子特性“双安定性”の寄与についての解析2017

    • Author(s)
      沈 宝國,和田 清二,山下(川野)絵美,小柳 光正,寺北 明久
    • Organizer
      日本動物学会第88回大会
  • [Presentation] Diversity of animal rhodopsins and their potentials for optical regulation of cell signaling2017

    • Author(s)
      Mitsumasa Koyanagi, Baoguo Shen, Takashi Nagata, Lanfang Sun, Seiji Wada, Satomi Kamimura, Eriko Kage-Nakadai, Akihisa Terakita
    • Organizer
      第9回光操作研究会
  • [Presentation] Detergent-free approach to spectral tuning mechanisms of long-wavelength-sensitive opsins in butterfly2017

    • Author(s)
      Tomoka Saito, Mitsumasa Koyanagi, Tomohiro Sugihara, Kentaro Arikawa, Akihisa Terakita
    • Organizer
      The 8th Asia & Oceania Conference on Photobiology
    • Int'l Joint Research
  • [Presentation] Diversity of non-visual animal opsins and their optogenetic applications2017

    • Author(s)
      Mitsumasa Koyanagi
    • Organizer
      日本比較生理生化学会第39回大会
    • Invited
  • [Presentation] Characterization of an opsin, peropsin, distributing to the spider brain and eyes2017

    • Author(s)
      Takashi Nagata, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      日本比較生理生化学会第39回大会
  • [Presentation] Investigation of the pineal photoresponse involving parapinopsin in the zebrafish under bright light conditions2017

    • Author(s)
      Seiji Wada, Baoguo Shen, Emi Kawano-Yamashita, Takashi Nagata, Satoshi Tamotsu, Mitsumasa Koyanagi, Akihisa Terakita
    • Organizer
      日本比較生理生化学会第39回大会
  • [Presentation] Investigation of amino acid residues involved in spectral tuning of butterfly long-wavelength-sensitive opsins2017

    • Author(s)
      Tomoka Saito, Mitsumasa Koyanagi, Tomohiro Sugihara, Kentaro Arikawa, Akihisa Terakita
    • Organizer
      日本比較生理生化学会第39回大会
  • [Remarks] 大阪市立大学大学院理学研究科生物地球系専攻 生体高分子機能学II研究室

    • URL

      http://www.sci.osaka-cu.ac.jp/biol/mphys/index.html

  • [Patent(Industrial Property Rights)] 特許権2017

    • Inventor(s)
      永田崇,小柳光正,寺北明久
    • Industrial Property Rights Holder
      永田崇,小柳光正,寺北明久
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-226272

URL: 

Published: 2018-12-17   Modified: 2022-02-28  

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