2017 Fiscal Year Final Research Report
Elucidation of the establishment mechanism of the secondary symbiosis and the artificial induction
| Project/Area Number |
26291073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KODAMA Yuuki 島根大学, 生物資源科学部, 准教授 (80582478)
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| Research Collaborator |
TANAKA Kenya
MURAKAMI Riko
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | ミドリゾウリムシ / 共生クロレラ / 二次共生 / 感染 / 細胞内共生 / 食胞 |
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| Outline of Final Research Achievements |
Paramecium bursaria is the only experimental system capable of inducing endosymbiosis between eukaryotic cells in synchronization with a large amount of cells. The genes of P. bursaria with markedly altered expression level in the presence or absence of symbiotic Chlorella cells were detected by RNAseq, and the antibodies raised for synthesized petides of the gene products were developed and used to know intracellular localization of the antigens. In addition, an antibody against the perialgal vacuole membrane, which plays an essential role to protect algae from lysosomal fusion in secondary symbiosis, and clarified timing of appearance of the antigen during infection process.
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| Free Research Field |
進化生物学
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