Generation and customization of plant artificial chromosomes in cereals.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292005
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Okayama University
• Principal Investigator: Murata Minoru 岡山大学, 資源植物科学研究所, 教授 (20166292)
• Co-Investigator(Kenkyū-buntansha): 長岐 清孝 岡山大学, その他部局等, 准教授 (70305481)
• Co-Investigator(Renkei-kenkyūsha): KASHIHARA Kazunari 岡山大学, 資源植物科学研究所, 技術専門職員 (60379807)
• Research Collaborator: SATO Kazuhiro 岡山大学, 資源植物科学研究所, 教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 植物人工染色体 / 染色体変異 / ゲノム再構成 / 配列特異的組換え / Cre/LoxP / トランスポゾン / イネ / オオムギ

Outline of Final Research Achievements

The objective of this study is to develop efficient methods for generation of plant artificial chromosomes that are able to accept a number of genes. The newly designed T-DNA construct (pDLHC) contained two LoxPs, one of which is transposable by a transposase. This transposition was designed to induce expression of the Cre recombinase gene, resulting in site-specific recombination between two separated LoxPs.　Among the progeny of rice plants transformed with pDLHC, one transformant was found to contain a large deletion on chromosome 10, which was caused by elimination of a DNA molecule circled by recombination between two LoxPs. If the centromere functions would be given to the circular DNA, artificial ring chromosomes would be generated.

Free Research Field

農学