Comprehensive analysis of archaeal transcriptional regulators in a hyperthermophile

2017 Fiscal Year Final Research Report

• Project/Area Number: 26292038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: Kanai Tamotsu 京都大学, 工学研究科, 講師 (10346083)
• Co-Investigator(Renkei-kenkyūsha): ATOMI Haruyuki 京都大学, 大学院工学研究科, 教授 (90243047)
• Research Collaborator: YOSHIDA Ko ; KANESEKI Tsuyoshi ; TANIMOTO Mitsuru ; ASLAM Mehwish ; GUNJI Ryoma ; YAMADA Masahiro
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: アーキア / 転写制御因子 / 超好熱菌

Outline of Final Research Achievements

We have identified a transcriptional repressors for gluconeogenic enzymes and intracellular polysaccharide synthase in the hyperthermophilic archaeon, Thermococcus kodakarensis. A transcriptional activator, Tar, may control the entire aromatic amino acid biosynthesis systems in this organism. A collaborative research with Ehime University conducted a three dimensional structural analysis of Tar. It was suggested that the novel Lrp/AsnC family transcriptional repressor is involved in the control of a asparaginase gene, with a possibility of a dynamic binding/dissociation mechanism with DNA. By genetic recombination of the transcriptional regulatory system of T. kodakarensis, strains which produce hydrogen with higher efficiency were developed.

Free Research Field

極限環境微生物学