Molecular mechanism and application of structural elements that govern carbohydrase-catalyzed transfer reaction

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292049
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA, Atsuo 北海道大学, (連合)農学研究科(研究院), 教授 (90186312)
• Co-Investigator(Kenkyū-buntansha): 奥山 正幸 北海道大学, (連合)農学研究科(研究院), 講師 (00344490)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 糖質酵素 / 糖転移反応 / 構造因子

Outline of Final Research Achievements

This project aims at identification and application of structural elements that regulate and improve the carbohydrase-catalyzed transfer activities by elucidating four promising phenomena, which we newly found. Results obtained are as follows: 1) we identify the structural elements to control the α-1,3-transfer reaction displayed by α-1,3-glucoside-transfer enzyme; 2) we reveal the reaction mechanism of the catalytic residue-mutated glycosidase that shows the high transfer yield; 3) a pore canal at the active site of glycosidase plays a role that supplies the catalytic water by study at the molecular level. 4) we analyze the polysaccharide-producing enzyme and identify its structural elements to contribute to the synthesis of oligosaccharides.

Free Research Field

応用生物化学