2018 Fiscal Year Final Research Report
Molecular mechanism underlying synchronization of circadian clock in higher plants to external environmental stimuli
| Project/Area Number |
26292051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Research Collaborator |
AOKI Setsuyuki
MITSUDA Nobutaka
TAKAGI Masaru
MIZUNO Takeshi
NOMOTO Yuji
OKA Haruka
KITAYAMA Miki
TAKATA Yusuke
KATO Shoko
IMAMURA Miyu
FURUKAWA Yoshiki
KOBAYASHI Masahide
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| Project Period (FY) |
2014-04-01 – 2019-03-31
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| Keywords | 概日時計 / 光情報伝達 / 温度入力 / 転写制御 |
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| Outline of Final Research Achievements |
Circadian clocks are intrinsic and entrainable mechanisms that generate biological rhythms with approximately 24-hours period. Resetting of circadian clocks play an essential role in order to synchronize phases of the biological rhythm with changes in diurnal day and night cycles. Light and changes in ambient temperature are two fundamental resetting cues. Focused on time-of-day-specific temperature effect on clock gene responses, it was found that PRR9, PRR7 & LNK1 are up-regulated in response to changes in temperatures specifically at late night phase. On the contrary, TOC1 is up-regulated response to changes in temperatures specifically at early night phase. These results suggest that the temperature-response is gated by circadian clock. Chromatin immunoprecipitation assays showed that PRR9, PRR7, LNK1 and LUX are direct targets of the nighttime repressor, suggesting that temperature signals feed into the clock transcriptional circuitry through the EC nighttime repressor.
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| Free Research Field |
分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
概日リズムの位相制御は一日周期で規則的に変動する外部環境を予測し、種々の生理機能について一日における特定の時間に最高のパフォーマンスを発揮できるよう生体の内生リズムを調節する上で非常に重要であると考えられる。本研究では暗期の入力信号に感受性を示す植物時計の性質を分子レベルで解析し、暗期の入力信号に感受性を示す転写抑制因子の機能を明らかにすることができた。この知見は概日時計が深く関与している植物の光周性花成や形態形成を含む成長様式全般を人工制御するための知識基盤となりうるだろう。
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