Analysis of mRNA export mechanism and applied mRNA biotechnology in eukaryote.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292053
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Masada Seiji 京都大学, 生命科学研究科, 准教授 (20260614)
• Co-Investigator(Kenkyū-buntansha): 井沢 真吾 京都工芸繊維大学, 工芸科学研究科, 准教授 (10273517)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: mRNA export / UAP56 / URH49 / structure / complex formation

Outline of Final Research Achievements

UAP56, a DEAD box RNA helicase, functions to mRNA export from the nucleus to the cytoplasm. In higher eukaryote, there is a paralog of UAP56, named URH49. Although their amino acid homology is more than 90%, they form different complex and export specific set of mRNAs. In this study, I analyzed the region to regulate the complex formation using molecular biology technique and the structural basis using　structural biology method. The results indicate that the region regulating the complex formation is within N domain of UAP56 and URH49 and that their structures are similar but the coordination of N and C domains were different between UAP56 and URH49 and the structure of linker region between N and C domains was also different.

Free Research Field

細胞分子生物学