Cellular and molecular bases for mediating health-promoting action of prebiotics and probiotics

2016 Fiscal Year Final Research Report

• Project/Area Number: 26292062
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Hokkaido University
• Principal Investigator: Sonoyama Kei 北海道大学, (連合)農学研究科(研究院), 准教授 (90241364)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: プレバイオティクス / プロバイオティクス / エキソソーム / microRNA / オルガノイド

Outline of Final Research Achievements

Our previous animal studies showed that indigestible oligosaccharides and Lactobacillus plantarum No.14 reduce allergic symptoms and obesity, respectively, whereas the cellular and molecular mechanisms remain unknown. We hypothesized that circulating exosomes are involved in the actions. The present study showed that anti-obese effect of L. plantarum No.14 is, at least in part, mediated by circulating exosomes. In addition, we found that supplementation of indigestible oligosaccharides and Lactobacillus strains alter the circulating microRNA profiles. Furthermore, we showed that intestinal organoids are useful for investigating the microRNA expression and exosome release in the intestinal epithelial cells.

Free Research Field

消化管生理学